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| Leitão,Cintia Camurça Fernandes; Costa,José Jackson Nascimento; Saraiva,Márcia Viviane Alves; Araújo,Valdevane Rocha; Figueiredo,José Ricardo; Hurk,Robert van den; Silva,José Roberto Viana. |
| This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pre-antral follicles; Caprine; Culture; Oocyte; Antrum formation; FSH-R. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100018 |
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| Cunha,E.V.; Costa,J.J.N.; Rossi,R.O.D.S.; Silva,A.W.B.; Passos,J.R.S.; Portela,A.M.L.R.; Pereira,D.C.S.T.; Donato,M.A.M.; Campello,C.C.; Saraiva,M.V.A.; Peixoto,C.A.; Silva,J.R.V.; Santos,R.P.. |
| The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caprine; Antrum formation; FSH-R; PCNA; Ultrastructure. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245 |
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